Sequences of 596 cDNA clones (565,977 bp) of Japanese flounder (Paralichthys olivaceus) leukocytes infected with hirame rhabdovirus

We have partially sequenced 785 sequences of 596 independent complementary DNA clones isolated from a cDNA library of Japanese flounder leukocytes inf ected with hirame rhabdovirus. These sequences consist of a total of 565,97 7 base pairs. The average size of the sequenced lengths was 721 bp. Of 596 clones, 386 (64.8%) were identified as previously reported genes by the BLA STN and BLASTX programs. About 30% of the identified clones could be recogn ized by only the BLASTX program. A total of 251 distinct genes were identif ied, and 181 of these genes are the first such genes reported from the tele ostei. Approximately 27% of the identified Japanese flounder genes appear t o be associated with cell division, cell structure or motility, and basic e nergy metabolism, 29% with gene or protein expression, and 17% with cell si gnaling, cell communication, and cell or organism defense. The most frequen tly identified expressed sequence tags of leukocytes of Japanese flounder w ere gelatinase b and ribosomal protein L23, which both had 1.34% prevalence .