Site-specific formation of abasic lesions in DNA

Authors
Coleman, RS Pires, RM
Citation
Rs. Coleman et Rm. Pires, Site-specific formation of abasic lesions in DNA, NUCLEOS NUC, 18(10), 1999, pp. 2141-2146
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
10
Year of publication
1999
Pages
2141 - 2146
Database
ISI
SICI code
0732-8311(1999)18:10<2141:SFOALI>2.0.ZU;2-0
Abstract
A method for the introduction of depurinated lesions in DNA is described, a nd is based on the formation of a covalent cross-link between an antisense oligonucleotide probe and the target DNA sequence followed by an unexpected ly mild thermal depurination.