Increased auxin efflux in the IAA-overproducing sur1 mutant of Arabidopsisthaliana: A mechanism of reducing auxin levels?

With the aim of investigating the mechanisms that maintain auxin homeostasi s in plants, we have monitored the net uptake and metabolism of exogenously supplied indole-3-acetic acid (IAA) and naphthalene-1-acetic acid (NAA) in seedlings of wild type and the IAA-overproducing mutant sur1 of Arabidopsi s thaliana. Tritiated IAA and NAA entered the seedling tissues within minut es and were mostly accumulated as metabolites, probably amino acid and suga r conjugates, The mutant seedlings were marked by a strong increase of [H-3 ]IAA metabolism and a reduction of the accumulation levels of both free [H- 3]IAA and [H-3]NAA. The same characteristics were observed in wild-type see dlings grown on 5 mu M picloram. We measured [H-3]NAA uptake in the presenc e of high concentrations of unlabeled NAA or the auxin efflux carrier inhib itor naphthylphthalamic acid (NPA). This abolished the difference in free [ H-3]NAA accumulation between the mutant or picloram-treated seedlings and w ild-type seedlings. These data indicated that active auxin efflux carriers were present in Arabidopsis seedling tissues. Picloram-treated seedlings an d seedlings of the IAA-overproducing mutant sur1 displayed increased auxin efflux carrier activity as well as elevated conjugation of IAA. There is pr evious evidence to suggest that conjugation is a means to remove excess IAA in plant cells. Here, we discuss the possibility of efflux constituting an additional mechanism for regulating free IAA levels in the face of an exce ss auxin supply.