The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811

Citation
M. Redenbach et al., The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811, PLASMID, 42(3), 1999, pp. 174-185
Citations number
49
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147619X → ACNP
Volume
42
Issue
3
Year of publication
1999
Pages
174 - 185
Database
ISI
SICI code
0147-619X(199911)42:3<174:TLPSOS>2.0.ZU;2-J
Abstract
The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the gene tically more studied Linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the he lp of an ordered cosmid contig for the complete 360-kb element, we have loc alized a 5439-bp fragment from the central region that confers autonomous r eplication in Streptomyces lividans. The minimal origin contains two overla pping ORFs which are separated from an AT-rich region which might correspon d to the replication start point. ORF1 revealed intensive similarity to a c lass of DNA-primase/helicases of actinophages and archael plasmids. In addi tion, we have identified a region in both terminal inverted repeats of SCP1 that shows significant homology to the transposable element Tn4811 located near the ends of the S. lividans 66 chromosome. (C) 1999 Academic Press.