The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811
M. Redenbach et al., The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811, PLASMID, 42(3), 1999, pp. 174-185
The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the gene
tically more studied Linear streptomycete replicons. Although the genetics
of SCP1 and its interaction with the host chromosome have been analyzed for
nearly three decades no information exists on its replication. With the he
lp of an ordered cosmid contig for the complete 360-kb element, we have loc
alized a 5439-bp fragment from the central region that confers autonomous r
eplication in Streptomyces lividans. The minimal origin contains two overla
pping ORFs which are separated from an AT-rich region which might correspon
d to the replication start point. ORF1 revealed intensive similarity to a c
lass of DNA-primase/helicases of actinophages and archael plasmids. In addi
tion, we have identified a region in both terminal inverted repeats of SCP1
that shows significant homology to the transposable element Tn4811 located
near the ends of the S. lividans 66 chromosome. (C) 1999 Academic Press.