R. Marsou et al., Distribution of Staphylococcus sciuri subspecies among human clinical specimens, and profile of antibiotic resistance, RES MICROB, 150(8), 1999, pp. 531-541
The distribution of three subspecies comprising Staphylococcus sciuri was d
etermined for a collection of 30 clinical isolates originating from Morocco
, the United Kingdom, and France. The sources of these isolates were princi
pally wounds, skin, and soft tissue infections. At the species level, the i
solates were identified according to biochemical characteristics and at the
subspecies level by the ribotyping technique. PCR analysis performed with
the 16S-23S ribosomal DNA intergenic spacer was less powerful for subspecie
s differentiation. S. sciuri subsp. sciuri was the most frequent subspecies
(21 isolates) found in the collection, whereas S. sciuri subsp. rodentium
(seven isolates) and S. sciuri subsp. carnaticus (two isolates) were less c
ommon. mecA or a mecA-related gene was detected by PCR and Southern blot in
all 30 S. sciuri isolates, supporting the suggestion that S. sciuri specie
s are the natural reservoir of the mecA gene. While the linA/linA' gene cod
ing for Lincomycin resistance was present in five isolates, an uncharacteri
zed gene for this resistance was suspected in seventeen other isolates. (C)
Editions scientifiques et medicales Elsevier SAS.