Deleted form of hepatocyte growth factor ameliorates the mortality rate ofsevere thermal injury in rats

Citation
H. Arisawa et al., Deleted form of hepatocyte growth factor ameliorates the mortality rate ofsevere thermal injury in rats, SURGERY, 126(5), 1999, pp. 925-932
Citations number
39
Categorie Soggetti
Surgery,"Medical Research Diagnosis & Treatment
Journal title
SURGERY
ISSN journal
00396060 → ACNP
Volume
126
Issue
5
Year of publication
1999
Pages
925 - 932
Database
ISI
SICI code
0039-6060(199911)126:5<925:DFOHGF>2.0.ZU;2-C
Abstract
Background. The modulating effects of the deleted form of hepatocyte growth factor (dHGF) on burn-induced mortality rates and hepatic protein synthesi s were studied in rats. Methods. Rats were anesthetized, subjected to a 40% full-thickness scald bu rn, and divided into 2 groups receiving dHGF and vehicle. Results. In normal rats, dHGF-treatment (1 mg/kg intravenously, twice daily )for 5 days increased the circulating plasma volume. In burned rats that we re receiving vehicle, the survival rate on day 23 after the burn was 27%. T he serum albumin levels were decreased and did not reverse to the normal le vels until day 23 after the burn. Serum aa-concentration in the injured rat s was increased, whereas serum levels of transferrin, total protein, and hi gh-density lipoprotein-cholesterol were decreased. The treatment of animals with dHGF (1 mg/kg intravenously, 3 times daily) for 3 days increased the survival rate on day 23 by 64%. In the animals treated with dHGF for 3 or 6 days, serum alpha(1)-, alpha(2)-, and beta-globulin concentrations were in creased by the dHGF treatment. The serum levels of albumin transferrin, tot al protein, and high-density lipoprotein-cholesterol reversed to normal lev els or higher: Conclusions. Our data show that dHGF treatment may attenuate the decrease o f the circulating plasma volume after burn and reduce a high risk of burn s hock. it is also indicated that dHGF accelerates synthesis of not only acut e-phase reactants but also other hepatic proteins such as albumin and trans ferrin on severe burn injury. These findings suggest that the appropriate u pregulation of hepatic protein synthesis induced by dHGF may accelerate the physiologic recovery process after thermal injury and contribute to amelio rating the burn-induced death.