A cell line (DF-1\J) expressing the envelope protein isolated from the ADOL
-Hc1 strain of the avian leukosis virus subgroup J (ALV-J) was used to anal
yze receptor interference to six different isolates of ALV-J as well as ALV
subgroups A-D. The traditional gag-specific enzyme-linked immunosorbent as
say (ELISA) as well as flow cytometry was used to evaluate viral infection.
The parental cell line (DF-1) was susceptible to all ALV subgroups tested
while the DF-1\J cell line was selectively resistant to the subgroup J isol
ates. The DF-IU cell line was resistant to infection by all six ALV-J isola
tes as determined using the gag-specific ELISA. There was no interference w
ith the other ALV subgroups (A-D) induced by the expression of the ADOL-Hcl
envelope. The ALV-J isolates used in this analysis are serologically disti
nct when analyzed by flow cytometry. Convalescent sera to ADOL-Hcl cross-re
acts with all of the ALV-J isolates tested; however, sera to HPRS-103 did n
ot bind to four of the six isolates. Based on the intensity and differentia
l binding of these antisera using flow cytometry, the six ALV-J isolates us
ed can be grouped into four categories. Thus the DF-1\J cell line is resist
ant to infection by a serologically and genetically diverse group of ALV-J
isolates and should be useful as a diagnostic tool. (C) 1999 Academic Press
.