Mw. Frampton et al., Effects of aqueous extracts of PM10 filters from the Utah Valley on human airway epithelial cells, AM J P-LUNG, 277(5), 1999, pp. L960-L967
Citations number
32
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
We hypothesized that the reduction in hospital respiratory admissions in th
e Utah Valley during closure of a local steel mill in 1986-1987 was attribu
table in part to decreased toxicity of ambient air particles. Sampling filt
ers for particulate matter < 10 mu m (PM10) were obtained from a Utah Valle
y monitoring station for the year before (year 1), during (year 2), and aft
er (year 3) the steel mill closure. Aqueous extracts of the filters were an
alyzed for metal content and oxidant production and added to cultures of hu
man respiratory epithelial (BEAS-2B) cells for 2 or 24 h. Year 2 dust conta
ined the lowest concentrations of soluble iron, copper, and zinc and showed
the least oxidant generation. Only dust from year 3 caused cytotoxicity (b
y microscopy and lactate dehydrogenase release) at 500 mu g/ml. Year 1 and
year 3, but not year 2, dust induced expression of interleukin-6 and -8 in
a dose-response fashion. The effects of ambient air particles on human resp
iratory epithelial cells vary significantly with time and metal concentrati
ons.