A. Tapparo et al., The multigene immunophilin family of Dictyostelium discoideum. Characterization of microsomal and mitochondrial cyclophilin isoforms, BIOCHIMIE, 81(10), 1999, pp. 943-954
The sequences of two cyclophilin (Cyp) isoforms from Dictyostelium discoide
um have been determined. cyp2 is expressed as a 197 amino acid protein, whi
ch contains a 22 amino acid-long signal sequence, characteristic of endopla
smic reticulum localization signals, and that is cleaved in the mature prot
ein. Mature Cyp2 has a molecular mass of 18 986 Da. The cyp3 gene encodes a
174 amino acid protein with a predicted molecular mass of 19 016 Da. Its s
equence reveals no targeting sequence. From the MS analysis of affinity-pur
ified cyclophilins from different subcellular compartments, we localized th
e previously described Cyp1 (Barisic K. et al., Dev. Genet. 12 (1991) 50-53
) in cytosol, Cyp2 in microsomes and Cyp3 in mitochondria, respectively. Th
e expression of cyp1 mRNA is constant during differentiation, whereas the m
RNA level of both cyp2 and cyp3 is regulated and decreases steadily during
development. (C) 1999 Societe francaise de biochimie et biologie moleculair
e / Editions scientitiques et medicales Elsevier SAS.