Stomatal morphology during acclimatization of tobacco plantlets to ex vitro conditions

Image analysis was used in studying stomatal morphology during acclimatizat ion of tobacco plantlets to ex vitro conditions. 45 d after transfer leaf a rea was 15 times, and total number of stomata per leaf four times increased . During acclimatization stomatal density was decreased considerably on bot h leaf sides, and was compensated by an increase in stomatal sizes, e.g., i n stomatal length and in stomatal area (both guard cells and pore), Elongat ion of stomata was increased indicating that the originally circular stomat a of in vitro plantlets were changed into elliptical ones in ex vitro accli matized plants.