Inhibitory effect of murine kidney extracts on the proliferation of murinemast cells

Authors
Murakami, M Kashiwakura, I Hayase, Y Takagi, Y
Citation
M. Murakami et al., Inhibitory effect of murine kidney extracts on the proliferation of murinemast cells, BIOL PHAR B, 22(11), 1999, pp. 1153-1157
Citations number
35
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
09186158 → ACNP
Volume
22
Issue
11
Year of publication
1999
Pages
1153 - 1157
Database
ISI
SICI code
0918-6158(199911)22:11<1153:IEOMKE>2.0.ZU;2-P
Abstract
We examined the effect of murine kidney extract (MKE) on time clonal growth of mast cells from murine peritoneal cells. Adding MKE resulted in a 40% i nhibition of colony formation of mast cells in a methylcellulose culture, a nd a 90% decrease in mast cell numbers and histamine content in mast cells in a liquid culture containing stem cell factor and interleukin-3. The mast cell inhibitory factors in MKE were heat sensitive proteins of approximate ly 560 and 24 kDa. These results suggest that MKE contains regulators that suppress the growth of murine mast cells and histamine synthesis.