Behaviour of pectinases under process conditions

Hydrolysis of pectic substances by pectinases were carried out under proces s conditions. Polymethylgalacturonase, polygalacturonase and pectinlyase ar e three enzymes studies in this regard. Pectin is used as the substrate for polymethylgalacturonase and pectinlyase whereas polygalacturonic acid was used as the substrate for polygalacturonase. The initial concentration was varied between 1.0 and 5.0 kg/m(3) for polymethylgalacturonase and polygala cturonase and between 4.0 and 8.0 kg/m(3) for pectinylase. Hydrolysis exper iments were carried out by varying initial substrate to enzyme ratio and re ducing groups formed are measured. The behaviour of pectinases during hydro lysis are studied from the plot of (reducing groups formed)/initial substra te vs. initial substrate to enzyme ratio. This study is helpful in predicti ng the amount of substrate and enzyme required to produce the required amou nt of reducing groups within a stipulated time.