Hydrolysis of pectic substances by pectinases were carried out under proces
s conditions. Polymethylgalacturonase, polygalacturonase and pectinlyase ar
e three enzymes studies in this regard. Pectin is used as the substrate for
polymethylgalacturonase and pectinlyase whereas polygalacturonic acid was
used as the substrate for polygalacturonase. The initial concentration was
varied between 1.0 and 5.0 kg/m(3) for polymethylgalacturonase and polygala
cturonase and between 4.0 and 8.0 kg/m(3) for pectinylase. Hydrolysis exper
iments were carried out by varying initial substrate to enzyme ratio and re
ducing groups formed are measured. The behaviour of pectinases during hydro
lysis are studied from the plot of (reducing groups formed)/initial substra
te vs. initial substrate to enzyme ratio. This study is helpful in predicti
ng the amount of substrate and enzyme required to produce the required amou
nt of reducing groups within a stipulated time.