Aprotinin recovery: Comparison between biospecific and peudobiospecific affinity adsorptions

Two adsorption techniques were studied as potential methods for the recover y of aprotinin: a biospecific adsorption (using immobilized trypsin and chy motrypsin as ligants) and a pseudobiospecific adsorption of aprotinin-tryps in complex onto IMAC matrix. These studies indicated that ionic strength ha s an important effect on aprotinin adsorption on immobilized trypsin, and t he pH was the most significant variable in aprotinin desorption from both i mmobilized enzymes. The aprotinin-trypsin complex adsorption onto the IMAC matrix was not as significantly affected by changes in pH as it was for cha nges in ionic strength. For the desorption step, both variables significant ly affected the complex recovery. However, the effect of ionic strength was markedly stronger for this desorption step.