Effects of chemical structure on the chromatographic retention characteristics of 5-alkyl-2 '-deoxyuridine derivatives. Application of porous graphitised carbon column

The retention of eleven deoxyuridine derivatives was determined on a porous graphitised carbon (PGC) column using methanol and water mixtures as eluen ts, Linear correlations were calculated between the log k values and the me thanol concentration in the eluent. The intercept (log k(0)) and slope (b) values were regarded as the best estimations of the retention capacity and the contact surface area of the deoxyuridines with the stationary phase. Bo th the slope and intercept values showed high variations suggesting that PG C can be succesfully used for the separation and quantitative determination of deoxyuridines. The retention of deoxyuridines significantly depends on the size of the substituents. Principal component analysis followed by non- linear mapping revealed that lipophilicity and electronic interactions of t he substituents also influence the retention of deoxyuridines on a PGC pack ing.