Jna. Tettey et al., The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes, CHEM-BIO IN, 123(2), 1999, pp. 105-115
The metabolism of ethidium bromide by isolated rat hepatocytes is significa
ntly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-me
thylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- a
nd 1.5-fold increase, respectively in the amount of the principal metabolit
e, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from
untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced b
y pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidiu
m glucuronide and a transient unidentified species, have been detected by H
PLC and are formed only by hepatocytes from animals pre-treated with 3-MC.
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