Quantitative assessment of Malaysian Plasmodium falciparum clones to schizontocidal drugs before and after cryopreservation

Thirty clones were obtained from five Malaysian Plasmodium falciparum isola tes using the limiting dilution method. These clones were then subjected to antimalarial testing using the modified in vitro microtechnique. The resul ts showed that ST 85/B3, GC/C10 and ST 85/A2 clones decreased their suscept ibilities to 19, 41 and 28% whilst ST 12/F8, ST 85/B3 and ST 85/B3 clones s howed increases of 6, 43 and 21%, respectively, against chloroquine, mefloq uine and quinine after cryopreservation. Further results also indicated tha t GC/B4, GC/B7, GC/C10, ST 85/A5, ST 85/ D3, ST 148/F8 clones did not show any change (up to 2 decimal places) against chloroquine, ST 12/D5, ST 12/E8 , ST 12/F8, ST 148/A5 clones against quinine after cryopreservation. They, however, maintained their original susceptibilities after cryopreservation. Copyright (C) 1999 S. Karger AG, Basel.