M. Otaki et al., Experimental orthotopic heart and bilateral lung transplantation completedwithout cardiopulmonary bypass, CHEST, 116(5), 1999, pp. 1360-1364
Citations number
8
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Introduction: Most experimental studies of orthotopic heart and lung graft
failure are complicated by an inability to eliminate the rejection-specific
inflammatory mediator from the cardiopulmonary bypass.
Methods: The following model was developed in our laboratory to investigate
the feasibility of performing an orthotopic heart and bilateral lung trans
plantation without performing a cardiopulmonary bypass. Nineteen transplant
s were attempted using 19 pairs of mongrel dogs. The recipient dog (mean we
ight, 23 kg) was anesthetized, and the ascending aorta, the superior vena c
ava (SVC), the inferior vena cava (IVC), and the main bronchus were dissect
ed. Then, the donor dog (mean weight, 20 kg) was anesthetized, and the hear
t and lung block was prepared and explanted from the chest under cardiopleg
ic arrest. A Gore-tex shunt (W. L. Gore; Flagstaff, AZ) was placed side-to-
side between the recipient IVC and SVC, and then the donor right atrium was
anastomosed to the Gore-tex shunt. The donor ascending aorta was anastomos
ed to the recipient ascending aorta with a partial clamp. On completion of
these anastomoses, the donor heart was reperfused by the recipient heart an
d allowed to beat. When hemodynamic conditions were stable with double hear
ts, the recipient SVC and IV were ligated just proximal to the venous anast
omosis and the recipient aorta was ligated proximal to the anastomotic site
. The recipient trachea was anastomosed to the donor trachea with an end-to
-end anastomosis. Finally, the recipient heart and lungs were removed from
the chest and the sternum was closed.
Results: Four of the 19 transplants failed. Three died due to left ventricu
lar dysfunction, and one died due to bleeding. Mean (+/- SD) ischemic time
was 67 +/- 11 min with a mean (+/- SD) anastomotic time of 54 +/- 12 min. T
he 15 survivors were hemodynamically stable with or without the minimal use
of inotropic support (dopamine, 2 to 3 mu g/kg/min) 6 h after grafting, wi
th normal cardiac output, satisfactory oxygenation, and normal wall motion.
The sternotomy was repaired without loss of cardiopulmonary function.
Conclusions: On the basis of our experiences the experimental model of orth
otopic heart and bilateral lung transplantation completed "off pump" can be
technically feasible without the loss of cardiac and pulmonary functions.