Optimization of an HPLC method for the separation of erythromycin and related compounds using factorial design

Authors
Harang, V Westerlund, D
Citation
V. Harang et D. Westerlund, Optimization of an HPLC method for the separation of erythromycin and related compounds using factorial design, CHROMATOGR, 50(9-10), 1999, pp. 525-531
Citations number
9
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
50
Issue
9-10
Year of publication
1999
Pages
525 - 531
Database
ISI
SICI code
0009-5893(199911)50:9-10<525:OOAHMF>2.0.ZU;2-G
Abstract
An HPLC method based on a Prodigy ODS2 column with a mobile phase containin g phosphate buffer, tetrabutylammonium hydrogen sulfate (TBA) and an aceton itrile gradient was optimized for the determination of erythromycin in dela yed-release capsules. A central composite face-centered (CCF) factorial des ign was used with the factors pH, concentration of TEA and gradient slope. The method could separate erythromycin A from 7 related erythromycins.