Mitomycin C in biological fluids: Comparison of disk and cartridge solid-phase extraction and quantitation by HPLC in human plasma and ultrafiltrate

The performance of particle-loaded disks and traditional, packed extraction columns is compared. In this study, solid-phase extraction and liquid chro matographic analysis of the anticancer drug mitomycin C are duplicated usin g two devices that contain chemically similar Cls bonded silica. The follow ing parameters are used to assess performance: linearity, recovery, precisi on, and lowest limit of detection. The extraction of mitomycin C from plasm a and ultrafiltrate using particle-loaded membranes achieves better precisi on, improved concentration ability, lower detection limits, and greater eff iciency. In addiction, elution of drug from the membrane adsorbent is effic iently accomplished using a small volume of mobile phase, eliminating analy te instability problems and the need for additional time-consuming evaporat ion.