Fertilization-induced activation of phospholipase C in the sea urchin egg

Citation
Bj. Rongish et al., Fertilization-induced activation of phospholipase C in the sea urchin egg, DEVELOP BIO, 215(2), 1999, pp. 147-154
Citations number
46
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENTAL BIOLOGY
ISSN journal
00121606 → ACNP
Volume
215
Issue
2
Year of publication
1999
Pages
147 - 154
Database
ISI
SICI code
0012-1606(19991115)215:2<147:FAOPCI>2.0.ZU;2-J
Abstract
Fertilization results in the biphasic activation of polyphosphoinositide-sp ecific phospholipase C (PLC) activity with an initial increase in activity coincident with the sperm-induced calcium transient, followed by a more sus tained increase prior to mitosis. Immunoprecipitation studies demonstrated that the gamma isoform of PLC is present in both the unfertilized and the f ertilized egg and contributes to the initial phase of PLC activation. ferti lization also resulted in translocation of a significant fraction of PLC-ga mma from the cytosol to the membrane compartment of the egg. (C) 1999 Acade mic Press.