A. Ghodsi et al., Systemic hyperosmolality improves beta-glucuronidase distribution and pathology in murine MPS VII brain following intraventricular gene transfer, EXP NEUROL, 160(1), 1999, pp. 109-116
Mucopolysaccharidosis VII, a classical lysosomal storage disease, is caused
by deficiency of the enzyme beta-glucuronidase. Central nervous system (CN
S) manifestations are severe with accumulations of storage vacuoles in all
cell types. Intraventricular gene transfer can lead to transduction of the
ependyma, with production and secretion of beta-glucuronidase into the cere
bral spinal fluid and underlying cortex resulting in reversal of disease pa
thology restricted to the periventricular areas. We tested if systemic hype
rosmolality would increase the distribution of beta-glucuronidase in brain
parenchyma after intraventricular virus injection. Mice were administered m
annitol, intraperitoneally, 20 days after gene transfer and 1 day prior to
sacrifice. Mannitol-induced systemic hyperosmolality caused a marked penetr
ation of beta-glucuronidase into the brain parenchyma. If mannitol was admi
nistered at the time of the intraventricular injection of virus, there was
penetration of vector across the ependymal cell layer, with infection of ce
lls in the subependymal region. This also resulted in increased beta-glucur
onidase activity throughout the brain. Sections of brains from beta-glucuro
nidase-deficient mice showed correction of cellular pathology in the subepe
ndymal region plus cortical structures away from the ventricular wall. Thes
e data indicate that virus-mediated gene transfer to the brain via the vent
ricles, coupled with systemic mannitol administration, can lead to extensiv
e CNS distribution of beta-glucuronidase with concomitant correction of the
storage defect. Our findings have positive therapeutic implications for th
e treatment of CNS disorders with gene transfer vectors and recombinant pro
teins. (C) 1999 Academic Press.