Psoralen in conjunction with UVA (PUVA) is perhaps the most effective treat
ment for psoriasis, It is, however, a risk factor for skin cancer in these
patients and there is a need to develop non-invasive assays reflective of t
reatment-induced DNA damage. We report here the assessment of two important
lesions, thymine dimer (T[]T) and 8-oxo-2'-deoxyguanosine (8-OHdG), in the
urine of psoriasis patients. It was found that, once corrected for urine c
oncentration, the psoriatic group had significantly higher (P < 0.0001) uri
nary levels of thymine dimers compared to the control group. No significant
differences in urinary 8-OHdG levels were noted between the psoriatic, ato
pic dermatitis and control groups. Therefore biomonitoring of therapy from
the very start with this simple and non-invasive assay could perhaps be an
effective measure of the risk involved with the treatment allowing optimiza
tion for minimal-risk therapy. (C) 1999 Federation of European Biochemical
Societies.