Isolation of protoplasts from in vitro-grown quince BA29 leaves

The aim of this research was to isolate protoplasts from in vitro-grown qui nce BA 29 leaves. Effects of various concentrations of cellulase and pectin ase and different incubation periods were evaluated. The most effective tre atment included (per ml) 50 units cellulase and 10 units pectinase, in the incubation medium and incubation for 21 h at 50 rpm in the dark at 27 degre es C, This treatment produced a protoplast yield of 8.2 X 10(7) gfw(-1) of which 90% were viable. Protoplast viability was influenced by length of the incubation period and ranged from about 40 to 100% among the various exper iments.