Analysis of vervain flavonoids by HPLC/diode array detector method. Its application to quality control

A reversed-phase HPLC procedure is proposed for the determination of seven flavonoids (luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsil ineol, and eupatorin) in vervain samples. A simple extractive technique was developed, involving only extraction with ethyl ether, which allowed the e limination of phenolic acids, yielding a sufficiently purified flavonoid fr action. All of the analyzed vervain samples showed a common flavonoid patte rn, in which hispidulin and jaceosidin were the major compounds and nepetin was the minor compound.