S. Kreft et al., Extraction of rutin from buckwheat (Fagopyrum esculentum Moench) seeds anddetermination by capillary electrophoresis, J AGR FOOD, 47(11), 1999, pp. 4649-4652
The content of the flavonoid rutin was determined in different milling frac
tions of buckwheat seeds and in buckwheat stems, leaves, and flowers. The e
xtraction was performed by using a solvent containing 60% of ethanol and 5%
of ammonia in water. The extracts were analyzed by capillary electrophores
is (running buffer of 50 mM berate (pH 9.3), 100 mM sodium dodecyl sulfate;
determination at 380 nm). In bran fractions the concentration of rutin was
131-476 ppm, and in flour fractions 19-168 ppm. On average, about 300, 100
0, and 46000 ppm of rutin were found in leaves, stems, and flowers, respect
ively. The results indicate that buckwheat could be an important nutritiona
l source of flavonoids, especially in countries with a low mean daily flavo
noid intake.