S. Rose et al., Fos expression within vasopressin-containing neurons in the suprachiasmatic nucleus of diurnal rodents compared to nocturnal rodents, J BIOL RHYT, 14(1), 1999, pp. 37-46
The underlying neural causes of the differences between nocturnal and diurn
al animals with respect to their patterns of rhythmicity have not yet been
identified. These differences could be due to differences in some subpopula
tion of neurons within the suprachiasmatic nucleus (SCN) or to differences
in responsiveness to signals emanating from the SCN. The experiments descri
bed in this article were designed to address the former hypothesis by exami
ning Fos expression within vasopressin (VP) neurons in the SCN of nocturnal
and diurnal rodents. Earlier work has shown that within the SCN of the diu
rnal rodent Arvicanthis niloticus, approximately 30% of VP-immunoreactive (
IR) neurons express Fos during the day, whereas Fos rarely is expressed in
VP-IR neurons in the SCN of nocturnal rats. However, in earlier studies, ra
ts were housed in constant darkness and pulsed with light, whereas Arvicant
his were housed in a light:dark (LD) cycle. To provide data from rats that
would permit comparisons with A. niloticus, the first experiment examined V
P/Fos double labeling in the SCN of rats housed in a 12:12 LD cycle and per
fused 4 h into the light phase or 4 h into the dark phase. Fos was signific
antly elevated in the SCN of animals sacrificed during the light compared t
o the dark phase, but virtually no Fos at either time was found in VP-IR ne
urons, confirming that the SCN of rats and diurnal Arvicanthis are signific
antly different in this regard. The authors also evaluated the relationship
between this aspect of SCN function and diurnality by examining Fos-IR and
VP-IR in diurnal and nocturnal forms of Arvicanthis. In this species, most
individuals exhibit diurnal wheel-running rhythms, but some exhibit a dist
inctly different and relatively nocturnal pattern. The authors have bred th
eir laboratory colony for this trait and used animals with both patterns in
this experiment. They examined Fos expression within VP-IR neurons in the
SCN of both nocturnal and diurnal A. niloticus kept on a 12:12 LD cycle and
perfused 4 h into the light phase or 4 h into the dark phase, and brains w
ere processed for immunohistochemical identification of Fos and VP. Both th
e total number of Fos-IR cells and the proportion of VP-IR neurons containi
ng Fos (20%) were higher during the day than during the night. Neither of t
hese parameters differed between nocturnal and diurnal animals. The implica
tions of these findings are discussed.