Chromatographic properties of reversed-phase stationary phases under pressure- and electro-driven conditions

Seven different reversed-phase (RP) stationary phases were examined under h igh-performance liquid chromatographic (pressure-driven, HPLC), and capilla ry electrochromatographic (electro-driven, CEC) conditions. Characterizatio n of the stationary phases was performed following well-established test pr ocedures providing a number of distinct column descriptors: hydrophobicity, hydrophobic selectivity and silanol activity. These parameters were used t o describe the behavior of the RP-columns under both HPLC and CEC condition s. It is shown that chromatographic characteristics of porous RP-phases gre atly depend on the mode of operation. By contrast, column descriptors of a non-porous viz. solid RP-phase material hardly differed for HPLC and CEC co nditions. (C) 1999 Published by Elsevier Science B.V. All rights reserved.