Arnebin-1 accelerates normal and hydrocortisone-induced impaired wound healing

Wound healing involves inflammation, cell proliferation, matrix deposition, and tissue remodeling. Interaction of different cells, extracellular matri x proteins, and their receptors are mediated by cytokines and growth factor s during wound healing. In this study, we have evaluated the effect of arne bin-1, a natural product isolated from Arnebia nobilis, on normal and impai red wound healing in cutaneous punch wound model. Arnebin-1 was applied top ically daily on wounds of hydrocortisone-treated or untreated animals. Arne bin-1 significantly accelerated healing of wounds with or without hydrocort isone treatment as revealed by a reduction in the wound width and gap lengt h compared with controls. Arnebin-1 treatment promoted the cell proliferati on, migration, and vessel formation to form a thick granulation tissue and re-epithelialization of the wounds, An increase in the synthesis of collage n, fibronectin and transforming growth factor-beta 1 was seen in arnebin-1- treated wounds compared with the untreated control. As transforming growth factor-beta 1 is known to enhance wound healing, and associated with the wo und healing defect in hydrocortisone-treated wounds, the enhanced expressio n of transforming growth factor-beta 1 at both translational and transcript ional level by arnebin-1 may be responsible for the enhancement of wound he aling during normal and impaired wound repair, These studies suggest that a rnebin-1 could be developed as a potent therapeutic agent for wound healing in steroid-impaired wounds.