Mixed-mode GPC used to control the reaction between N,N-dimethylpropylene,-1,3-diamine and triglycerides

Gel permeation chromatography (GPC) was utilised to control the progress of the reaction between N,N-dimethylpropylene-1,3-diamine (DMPDA) and triglyc erides (fats). As per "ideal size exclusion mode", the sample is separated into triglycerides (TGC),;diglycerides (DGC), monoglycerides (MGC), and gly cerine (GC),as the component groups with sufficiently different molecular w eights. Moreover, the separation was obtained for MGC and amidoamines (AA), i.e. the reaction products, despite the fact that their molecular weights are the same in practice (Mw=348 and 358, respectively). The increased rete ntion time for amidoamines was assumed to be accounted for by the additiona l adsorption effects in "adsorption mode". Resulting from the overlapping o f two types of chromatographic separation mechanisms, an analytical method was obtained which could be called "mixed mode" conditions. This method allows for the control of the amidolysis reaction of fats at it s intermediate stages. The method is applicable for the respectively narrow distribution of molecular weights of triglycerides, which is the case in l ard, sunflower oil and/or rapeseed oil.