Wound healing involves matrix deposition, angiogenesis, and new tissue
growth. Cellular activity during healing is related to tissue oxygen
levels. Since wound healing requires oxygen, the purpose of this study
was to investigate the effects of hyperbaric oxygen (HBO) on cells in
volved in wound healing. Cultured endothelial cells and fibroblasts we
re exposed to HBO. The effect of varied partial pressure, oxygen satur
ation, and duration and frequency of exposure to HBO on cell prolifera
tion was determined by H-3-labeled thymidine incorporation, HBO causes
an increase in the partial pressure of oxygen in the medium of cultur
ed cells, leading to increased endothelial cell and fibroblast prolife
ration. Increased endothelial cell proliferation occurred after 15 min
of HBO. Fibroblasts required 120 min of HBO to produce a response. A
second exposure to HBO on the same day produced no additional increase
in cell proliferation. A 120-min HBO exposure stimulated fibroblast p
roliferation for 72 h after the exposure. An increase in pressure from
2.4 to 4.0 atmospheres absolute did not enhance the proliferative res
ponse. These studies begin to elucidate the effects of HBO on cells in
volved in wound healing and establish a scientific foundation upon whi
ch to develop more efficacious and cost-effective HBO therapeutic prot
ocols.