A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions

A system for studying the early growth response of Streptococcus gordonii t o environmental stimuli has been developed. A reporter gene, encoding a-amy lase, has been integrated into an rRNA operon to monitor changes in cellula r physiology associated with the initiation of growth. Two such strains wit h single integrants have been characterized during the transition from lag phase to exponential growth. Synthesis of the reporter is correlated to gro wth initiation in both strains, and the reporter enzyme is detectable with sufficient sensitivity. Comparison of the expression profiles of the two rm operons containing the reporter gene suggests that they are differentially expressed over the course of growth. (C) 2000 Published by Elsevier Scienc e B.V. All rights reserved.