E. Harada et al., Effect of interleukin-1 beta on cardiac hypertrophy and production of natriuretic peptides in rat cardiocyte culture, J MOL CEL C, 31(11), 1999, pp. 1997-2006
This study was designed to examine the effects of interleukin-1 beta (IL-1
beta) on myocyte (MC) hypertrophy and the production of A-type natriuretic
peptide (ANP) and B-type natriuretic peptide (BNP) in rat ventricular cardi
ocyte culture, and to investigate the role of nonmyocyte (NMC) in this proc
ess. We examined the effects of IL-1 beta on the production of ANP and BNP
in comparison with the effects of endothelin-1 (ET-1) by using two types of
neonatal rat cardiocyte culture; MC-enriched culture and MC-NMC coculture.
In the MC-enriched culture, the increase in secretion of ANP and BNP was s
mall in treatment with IL-1 beta (1000 pg/ml), while ET-1 (10 nM) markedly
augmented the secretion of ANP and BNP. In the MC-NMC coculture, IL-1 beta
and ET-1 each significantly augmented the secretion of ANP and BNP. The deg
ree of the increase of ANP and BNP was equivalent between IL-1 beta and ET-
1. As for the morphological changes of MCs, IL-1 beta induced the star-shap
ed MC hypertrophy characterized by elongation and pointed edges only in the
MC-NMC coculture, while ET-1 induced the MC hypertrophy characterized by s
hapes of squares, triangles or circles in both cultures. This study shows t
hat IL-1 beta induces unique cardiac hypertrophy and the marked secretion o
f ANP and BNP, and that NMC is indispensable when treated with IL-1 beta. (
C) 1999 Academic Press.