Interleukin-17 stimulates the expression of I kappa B alpha mRNA and the secretion of IL-6 and IL-8 in glioblastoma cell lines

Interleukin-17 (IL-17) has been characterized as a proinflammatory cytokine produced by CD4(+) activated memory T cells. In an effort to elucidate the biological effects of IL-17 in glial cells, we investigated the ability of this cytokine in order to activate nuclear factor (NF)-kappa B, which is b eing discussed as one of the most important transcription factors in the re gulation of neuronal and glial cell function. Activation of NF-kappa B invo lves the degradation of its cytoplasmatic inhibitor I kappa B-alpha, which allows the nuclear translocation of NF-kappa B, and ensures transcriptional activation of genes including I kappa B-alpha itself. Using a competitive RT-PCR, we examined the IL-17-induced I kappa B-alpha mRNA expression in gl ioblastoma cells, and we examined IL-17 up-regulated I kappa B-alpha mRNA e xpression in a dose- and time-dependent fashion with a maximum time between 1 and 3 h. This induction could be inhibited by Calphostin C (proteinkinas e C inhibitor) and genistein (tyrosine kinase inhibitor). After 60 min of I L-17 stimulation, a degradation of the I kappa B-alpha protein was detectab le. Furthermore, IL-17 stimulated the secretion of IL-6 and IL-8 in glial c ells, and IL-17 and IL-1 beta in combination showed a superadditive effect. We suggest IL-17 to play a role as an immune factor, possibly involved in complex pathophysiological interactions of neurodegenerative diseases. (C) 1999 Elsevier Science B.V. All rights reserved.