Comparison of derivative spectrophotometric and liquid chromatograpic methods for the determination of omeprazole in aqueous solutions during stability studies

A first derivative spectrophotometric method was developed for the determin ation of omeprazole in aqueous solutions during stability studies. The deri vative procedure was based on the linear relationship between the omeprazol e concentration and the first derivative amplitude at 313 nm. The first der ivative spectra was developed between 200 and 400 nm (Delta gamma = 8). Thi s method was validated and compared with the official high-performance liqu id chromatography (HPLC) method of the USP. It showed good linearity in the range of concentrations studied (10-30 mu g ml(-1)), precision (repeatabil ity and inter-day reproducibility), recovery and specificity in stability s tudies. It also seemed to be 2.59 times more sensitive than the HPLC method . These results allowed to consider this procedure as useful for the rapid analysis of omeprazole in stability studies since there was no interference with its decomposition products. (C) 1999 Elsevier Science B.V. All rights reserved.