CLONING AND SEQUENCING OF A SPECIES-SPECIFIC NUCLEOTIDE FRAGMENT OF BORRELIA-BURGDORFERI SENSU-STRICTO, WHICH IS REPEATED IN SEVERAL PLASMIDS OF THE SPECIES

Authors
MISONNE MC SCHUTTLER M DERNELLE JM DEKESEL M HOET PP
Citation
Mc. Misonne et al., CLONING AND SEQUENCING OF A SPECIES-SPECIFIC NUCLEOTIDE FRAGMENT OF BORRELIA-BURGDORFERI SENSU-STRICTO, WHICH IS REPEATED IN SEVERAL PLASMIDS OF THE SPECIES, FEMS microbiology letters, 150(1), 1997, pp. 157-164
Citations number
23
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
150
Issue
1
Year of publication
1997
Pages
157 - 164
Database
ISI
SICI code
0378-1097(1997)150:1<157:CASOAS>2.0.ZU;2-F
Abstract
Among the etiological agents of Lyme disease, Borrelia burgdorferi sen su stricto strains carry a 16 kb plasmid, which did not hybridize to p lasmids of B. garinii and B. afzelii strains, A 1271 bp DNA fragment o f the 16 kb plasmid was cloned. It hybridized to several plasmids of t his species (16, 27 and 55 kb). Sequencing of the cloned insert reveal ed a 327 bp ORF coding for a 14 kDa protein of unknown function, which could be expressed in E. coil. This ORF, conserved among B. burgdorfe ri sensu stricto strains, was carried by the same three plasmids.