Length-mass relationships for freshwater macroinvertebrates in North America with particular reference to the southeastern United States

Estimation of invertebrate biomass isa critical step in addressing many eco logical questions in aquatic environments. Length-dry mass regressions are the most widely used approach for estimating benthic invertebrate biomass b ecause they are faster and more precise than other methods. A compilation a nd analysis of length-mass regressions using the power model, M (mass) = a L (length)(b), are presented from 30 y of data collected by the authors, pr imarily from the southeastern USA, along with published regressions from th e rest of North America. A total of 442 new and published regressions are p resented, mostly for genus or species, based on total body length or other linear measurements. The regressions include 64 families of aquatic insects and 12 families of other invertebrate groups (mostly molluscs and crustace ans). Regressions were obtained for 134 insect genera (155 species) and 153 total invertebrate genera (184 species). Regressions are provided for both body length and head width for some taxa. In some cases, regressions are p rovided from multiple localities for single taxa. When using body length in the equations, there were no significant differences in the mean value of the exponent b among 8 insect orders or Amphipoda. The mean value of b for insects was 2.79, ranging from only 2.69 to 2.91 among orders. The mean val ue of b for Decapoda (3.63), however, was significantly higher than all ins ects orders and amphipods. Mean values of a were not significantly differen t among the 8 insect orders and Amphipoda, reflecting considerable variabil ity within orders. Reasons for potential differences in b among taxa are ex plained with hypothetical examples showing how b responds to changes in lin ear dimensions and specific gravity. When using head width as the linear di mension in the power model the mean value of b was higher (3.11) than for b ody length and more variable among orders (2.8-3.3). Values of b for Epheme roptera (3.3) were significantly higher than those for Odonata, Megaloptera , and Diptera. For those equations in which ash-free dry mass was used, % a sh varied considerably among functional feeding groups (3.3-12.4%). Percent ash varied from 4.0% to 8.5% among major insect orders, but was 18.9% for snails (without shells). Family-level regressions also are presented so tha t they can be used when generic equations are unavailable or when organisms are only identified to the family level. It is our intention that these re gressions be used by others in estimating mass from linear dimensions but p otential errors must be recognized.