H. Wessells et Sk. Williams, Endothelial cell transplantation into the corpus cavernosum: Moving towards cell-based gene therapy, J UROL, 162(6), 1999, pp. 2162-2164
Purpose: As a first step toward a cell based gene therapy for erectile dysf
unction, we transplanted fluorescently labeled autologous microvessel endot
helial cells (MVEC) into the rat corpus cavernosum.
Materials and Methods: MVEC were isolated fr om the epididymal fat pad, lab
eled with the membrane intercalating dye PKH 26, and injected into the corp
us cavernosum. Two to 15 days aff;er transplantation the penises were remov
ed, cryosectioned, and examined under epifluorescent and phase contrast mic
roscopy.
Results: In 7 consecutive animals transplanted fluorescent cells were ident
ified in the corpora cavernosa. Bilateral distribution was noted in each an
imal, and staining with ED1 determined that the fluorescence was not due to
engulfment of the MVEC by phagocytic cells.
Conclusion: Transplanted endothelial cells adhere and persist in the corpor
al sinusoids and provide a rationale for cell-based gene therapy in the pen
is.