Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C

Authors
Hadaschik, D Klein, M Zimmermann, H Eggers, HJ Nelsen-Salz, B
Citation
D. Hadaschik et al., Dependence of echovirus 9 on the enterovirus RNA replication inhibitor 2-(alpha-hydroxybenzyl)-benzimidazole maps to nonstructural protein 2C, J VIROLOGY, 73(12), 1999, pp. 10536-10539
Citations number
24
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
73
Issue
12
Year of publication
1999
Pages
10536 - 10539
Database
ISI
SICI code
0022-538X(199912)73:12<10536:DOE9OT>2.0.ZU;2-B
Abstract
HBB [2-(alpha-hydroxybenzyl)-benzimidazole] selectively inhibits RNA synthe sis of most enteroviruses. However, isolation of HBB-dependent variants is possible. Sequence analysis and characterization of recombinant, viruses re vealed that HBB dependence maps to the nonstructural protein 2C. A single p oint mutation at position C4782U is sufficient to establish the HBB-depende nt phenotype in our echovirus 9 model.