The effect of immunization with porins on gut pathophysiological response in rats infected with Salmonella typhimurium

Attachment of Salmonella typhimurium to epithelial surfaces elicit signific ant alterations in different cell signalling events which lead to the devel opment of disease. The present investigation was conducted to evaluate the effect of immunization of rats with porins, on gut physiologic markers foll owing challenge with S. typhimurium. Male albino Wistar rats were immunized with purified porins and challenged by intragastric infection with S. typh imurium. Electrolyte transport, levels of different second messengers and i nflammatory mediators were studied. A net absorption of transepithelial flu xes of Na+ and Cl- in immunized-challenged group and secretion in infected group was found. Ca2+ and 3-O-methyl-D-glucose fluxes did not show any chan ge. Significant increase in the levels of [Ca2+](i), cAMP, membrane form of protein kinase C, prostaglandins, NADPH oxidase, Glucose-6-phosphate dehyd rogenase, 6-phosphogluconate dehydrogenase, total oxygen free radicals, rea ctive nitrogen intermediates, citrulline and lipid peroxidation was found i n the infected group. However, in the immunized-challenged group, the value s of all the parameters were found to be almost the same as that of control as well as immunized groups. Na+, K+-ATPase and calmodulin levels were una ltered in all the groups of animals. The results of this study thus suggest that immunization of rats with purified Salmonella porins followed by subs equent challenge with the organism might be helpful for the prevention of m ultiple physiologic derangements in isolated ideal cells.