Ci. Petersen et Lj. Defelice, Ionic interactions in the Drosophila serotonin transporter identify it as a serotonin channel, NAT NEUROSC, 2(7), 1999, pp. 605-610
Serotonin transporters (SERTs) are targets for drugs such as Prozac that in
crease serotonin (5HT) levels by blocking 5HT reuptake. Although SERTs satu
rate in the micromolar range, synaptic 5HT may exceed 1 mM. To examine SERT
's response to high 5HT concentrations, we expressed Drosophila SERT (dSERT
) in Xenopus oocytes and found that transport continued to increase with co
ncentration up to 0.3 mM 5HT. As 5HT is a monovalent cation, its entry thro
ugh an ion channel in SERT might explain uptake at high concentrations. We
therefore investigated dSERT using traditional ion channel methods, includi
ng mole-fraction experiments under voltage clamp. We propose that SERTs may
function as 5HT-permeable channels, and that this mechanism may be importa
nt for clearance of the neurotransmitter at high concentrations.