Sun exposure histories were obtained from a series of patients age 35 or yo
unger following diagnosis and removal of a basal cell carcinoma (BCC). The
DNA was extracted from tumor biopsy samples derived from BCC of 10 patients
who reported that they did not use sunscreens during youth (age 18 or youn
ger) and 10 patients who routinely employed sunscreens during this age peri
od. Exons 5-9 of the p53 gene were then amplified in three fragments from t
hese samples using a nested polymerase chain reaction (PCR) approach and sc
reened for mutations using an RNA heteroduplex assay. All PCR products disp
laying evidence of a mutation were sequenced. It was found that 6 of the 10
patients who were not routine sunscreen users displayed mutations in these
p53 exons, All of the mutations were located at dipyrimidine sites, five o
f the six were C-->T transitions and one mutation was a tandem double mutat
ion, consistent with a role for solar UVB in BCC formation. In contrast, on
ly one p53 mutation was detected in the group of 10 patients who routinely
employed sunscreens during childhood and adolescence, Hence, a significantl
y (P = 0.029) lower level of p53 mutations was detected in the BCC obtained
from sunscreen users compared with tumors derived from nonusers. These fin
dings suggest that the mechanisms involved in the etiology of skin carcinog
enesis differ in sunscreen users compared with people who did not routinely
employ sunscreens, These data are also indicative of a protective effect a
ssociated with sunscreen use against the formation of p53 mutations. It is
possible that the patients who were diagnosed with BCC despite their use of
sunscreens possessed a genetic susceptibility for skin cancer formation an
d developed BCC through a p53-independent pathway, Alternatively, solar UVA
wavelengths, that were generally not blocked by the suncare products emplo
yed by the sunscreen users, may have played a significant role? in BCC deve
lopment through induction of a mutation(s) in an oncogene and/or a tumor su
ppressor gene, other than p53, for these patients.