Characterization of enzymatically induced degradation of articular cartilage using high frequency ultrasound

Ultrasound may provide a quantitative technique for the characterization of cartilage changes typical of early osteoarthrosis. In this study, specific changes in bovine articular cartilage were induced using collagenase and c hondroitinase ABC, enzymes that selectively degrade collagen fibril network and digest proteoglycans, respectively. Changes in cartilage structure and properties were quantified using high frequency ultrasound, microscopic an alyses and mechanical indentation tests. The ultrasound reflection coeffici ent of the physiological saline-cartilage interface (R-1) decreased signifi cantly (-96.4%, p < 0.01) in the collagenase digested cartilage compared to controls. Also a significantly lower ultrasound velocity (-6.2%, p < 0.01) was revealed after collagenase digestion. After chondroitinase ABC digesti on, a new acoustic interface at the depth of the enzyme penetration front w as detected. Cartilage thickness, as determined with ultrasound, showed a h igh, linear correlation (R = 0.943, n = 60, average difference 0.073 mm (4. 0%)) with the thickness measured by the needle-probe method. Both enzymes i nduced a significant decrease in the Young's modulus of cartilage (p < 0.01 ). Our results indicate that high frequency ultrasound provides a sensitive technique for the analysis of cartilage structure and properties. Possibly ultrasound may be utilized in vivo as a quantitative probe during arthrosc opy.