Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones toflavones

Authors
Akashi, T Fukuchi-Mizutani, M Aoki, T Ueyama, Y Yonekura-Sakakibara, K Tanaka, Y Kusumi, T Ayabe, S
Citation
T. Akashi et al., Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones toflavones, PLANT CEL P, 40(11), 1999, pp. 1182-1186
Citations number
26
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
00320781 → ACNP
Volume
40
Issue
11
Year of publication
1999
Pages
1182 - 1186
Database
ISI
SICI code
0032-0781(199911)40:11<1182:MCABCO>2.0.ZU;2-J
Abstract
Cytochrome P450 cDNAs, AFNS2 and TFNS5, were isolated from snapdragon and t orenia petal cDNA libraries, respectively, based on the sequence homology w ith Licorice CYP93B1 cDNA encoding (2S)-flavanone 2-hydroxylase. They were expressed in yeast and identified to encode flavone synthase II catalyzing direct conversion of flavanones to flavones probably via 2-hydroxyflavanone s.