HYALURONAN-INDEPENDENT LODGMENT OF CD44(-CELLS IN LYMPHOID ORGANS() LYMPHOMA)

We previously found that monoclonal antibodies (MAbs) directed against the constant region of the CD44 molecule block lymph node infiltratio n of a mouse LB T-cell lymphoma, suggesting a role for th is glycoprot ein in the LB cell dissemination process. In the present study, we inv estigated whether LB cells in the local tumor must undergo a change in the CD44 phenotype to be able to migrate to and invade the remote lym ph nodes, and if hyaluronic acid (HA), the principal ligand of activat ed CD44, functions as a mediator in this process. We compared the in v ivo behavior of a non-HA-binder LB cell line with that of a constituti ve HA-binder HA9 subline. Our results show that the lymphoid organ-inf iltrating LB cells express similar levels of pan-CD44 and V4- and V6-c ontaining CD44 variants, as the corresponding cells in the local growt h and the cultured LB cells. The tested CD44 phenotype of HA9 cells al so remained unchanged during the metastatic process. Even after lymph node infiltration, LB cells remained incapable of binding HA, whereas the HA9 cells retained an efficient HA-binding capacity. The constitut ive HA-binder HA9 cells that expressed an approximately 10-fold higher level of pan-CD44 than did the parental LB cells, as well as an eleva ted level of the V4 and V6 exon products formed a local tumor and inva ded both lymph nodes and spleen, as did the parental LB cells, albeit at a much slower rate. Our finding indicates that there is no direct c orrelation between the amount of CD44 expressed on the cell surface, t he HA-binding capacity and tumorigenicity. Moreover, interaction with HA is not obligatory for LB cell localization in the lymphoid organs, and a tight cell-HA interaction, as observed in HA9 cells, does not pr event tumor cell dissemination, although it may retard tumor spread. ( C) 1997 Wiley-Liss, Inc.