DNA binding studies of a solvatochromic fluorescence probe 3-methoxybenzanthrone

Citation
X. Yang et al., DNA binding studies of a solvatochromic fluorescence probe 3-methoxybenzanthrone, SPECT ACT A, 55(13), 1999, pp. 2719-2727
Citations number
29
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN journal
13861425 → ACNP
Volume
55
Issue
13
Year of publication
1999
Pages
2719 - 2727
Database
ISI
SICI code
1386-1425(199911)55:13<2719:DBSOAS>2.0.ZU;2-G
Abstract
A fluorescence probe of 3-methoxybenzanthrone (MBA) exhibits significant so lvatochromic characteristics correlated with the polarity of solvents. The interaction of the solvatochromic fluorescence probe with calf thymus DNA ( ct-DNA) has been investigated. In the presence of ct-DNA the fluorescence o f MBA is strongly quenched with a blue-shift of emission peak and a hypochr omism in absorption spectra. The absorption spectral fluorescence quenching and fluorescence polarization experiments show that the MBA molecule as an intercalator is inserted into the base-stacking domain of the ct-DNA doubl e helix, and the interaction of the nucleobases with the MBA molecule cause s quenching of fluorescence and hypochromism in the absorption spectra, The intrinsic binding constant and the binding site number were determined to be 1.70 x 10(5) mol l(-1) in base pairs and six, respectively. The I-0/I ve rsus [ct-DNA] plot shows linear relationship in the range covering 4.3 x 10 (-7) - 1.02 x 10(-4) mol l(-1) in base pairs which can be used for ct-DNA d etermination. The limit of detection was found to be 4.3 x 10(-7) mol l(-1) in base pairs (0.5 mu g ml(-1)). (C) 1999 Elsevier Science B.V. All rights reserved.