RAPID SEPARATION OF ENANTIOMERS IN PERFUSION CHROMATOGRAPHY USING A PROTEIN CHIRAL STATIONARY-PHASE

For the first time, enantiomer separation was performed using a polyme ric flow-through-type chromatographic support (POROS). Bovine serum al bumin (BSA) covalently bound to POROS was employed as the chiral stati onary phase. Using flow-rates of up to 10 ml/min, enantiomer separatio n of all investigated compounds, a variety of amino acid derivatives a nd drugs, could be achieved within a few minutes at medium efficiencie s typical for protein chiral stationary phases. It could be shown that the loss in resolution R-s with increasing velocity, u, of the mobile phase was lower than predicted by the theory for conventional support s, but not independent of the velocity, u, as expected by perfusion ch romatographic theory.