LIQUID-CHROMATOGRAPHY OF SPIRAMYCIN ON POLY(STYRENE-DIVINYLBENZENE)

An isocratic liquid chromatographic method is described using as stati onary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m 1000 Angstrom). The mobile phase is acetonitrile-0.2 M potassium pho sphate buffer pH 9.0-water (37:5:up to 100, v/v/v), delivered at a flo w-rate of 1.0 ml/min. UV detection is performed at 232 nm. Full factor ial design was applied to evaluate the robustness of the method. Quant itative results of a number of commercial samples are presented.