Neutralization of the hemorrhagic activity of Bothrops and Lachesis snake venoms by a monoclonal antibody against mutalysin-II

Authors
Estevao-Costa, MI Martins, MS Sanchez, EF Diniz, CR Chavez-Olortegui, C
Citation
Mi. Estevao-costa et al., Neutralization of the hemorrhagic activity of Bothrops and Lachesis snake venoms by a monoclonal antibody against mutalysin-II, TOXICON, 38(1), 2000, pp. 139-144
Citations number
13
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICON
ISSN journal
00410101 → ACNP
Volume
38
Issue
1
Year of publication
2000
Pages
139 - 144
Database
ISI
SICI code
0041-0101(200001)38:1<139:NOTHAO>2.0.ZU;2-G
Abstract
One mAb reactive with mutalysin-II, a hemorrhagic metalloproteinase isolate d from Lachesis muta muta venom, was produced in mice immunized with L. in. muta venom, Indirect ELISA was employed to compare the antigenic cross-rea ctivity among the venoms from Bothrops snakes. The mAb anti-mutalysin-II ef ficiently neutralized the hemorrhagic effect of both mutalysin-II and L. nl . muta crude venom. Furthermore, the mAb were crossreactive with B. alterna tus, B. atrox, B, itapetinigae, B. jararaca and B. neuwiedii and showed var iable potencies in neutralizing the hemorrhagic activity of several bothrop ic venoms. (C) 1999 Elsevier Science Ltd. All rights reserved.