The quantification of pollutant loads from agricultural non-point source po
llution is difficult because: (1) there is no direct means of continuously
recording pollutant concentration; (2) and available human resources and fu
nds always limit sampling. For those reasons, sampling strategy and polluta
nt concentration interpolation methods are essential to estimate pollutant
loads. In this study, a method was tested to evaluate pollutant loads using
daily flow volume and daily mass load regression for nitrate-nitrogen, ort
hophosphate-phosphorous and atrazine. The method worked well for nitrate-ni
trogen, but generally did not work well for either orthophosphate-phosphoro
us or atrazine. Nitrate-nitrogen load was calculated for several tile-monit
oring stations based on this method. Results were compared with volume inte
rpolation methods; the difference between the two methods is not significan
t at the 95% level of confidence. This method can provide a reliable estima
te of nitrate-nitrogen loads where limited samples are available. This meth
od was not successful with orthophosphate-phosphorous or atrazine.