Induction of systemic and mucosal antibody responses in mice immunized intranasally with aluminium-non-adsorbed diphtheria toroid together with recombinant cholera toxin B subunit as an adjuvant
M. Isaka et al., Induction of systemic and mucosal antibody responses in mice immunized intranasally with aluminium-non-adsorbed diphtheria toroid together with recombinant cholera toxin B subunit as an adjuvant, VACCINE, 18(7-8), 1999, pp. 743-751
Nasal mucosal immunization is very attractive for vaccination to prevent va
rious bacterial and viral infectious diseases because of induction of syste
mic and mucosal immune responses. The aim of the present study was to inves
tigate the possibility of changing the immunization procedure of diphtheria
toxoid (D-T) from intramuscular or subcutaneous injection to intranasal ad
ministration. Intranasal immunization with aluminium-non-adsorbed diphtheri
a toroid (nD(T)) together with recombinant cholera toxin B subunit (rCTB, 1
0 mu g) induced, at a concentration of 5 Lf, high levels of serum DT(-)spec
ific IgG antibody responses and high or moderate levels of the specific IgA
antibody responses in all mice and only a slight level of the specific IgE
antibody responses in some mice. Furthermore, sufficiently high diphtheria
antitoxin titres more than 0.1 international units (IU) ml(-1) were obtain
ed from mice which showed high levels of serum D-T-specific IgG antibody re
sponses. Under the same experimental conditions, induction of significant l
evels of mucosal D-T-specific IgA antibody responses occurred in the nasal
cavity, the lung, the saliva and vaginal secretions and the small and large
intestines of all mice, although there were different titres between indiv
idual mice. Similar results were also obtained with rCTB-specific serum IgG
and IgA and mucosal IgA antibody responses; serum rCTB-specific IgE antibo
dy titres were not detected. These results show that intranasal administrat
ion of nD(T) with rCTB must be a very useful means for vaccination against
diphtheria. (C) 1999 Elsevier Science Ltd. All rights reserved.