Induction of systemic and mucosal antibody responses in mice immunized intranasally with aluminium-non-adsorbed diphtheria toroid together with recombinant cholera toxin B subunit as an adjuvant

Citation
M. Isaka et al., Induction of systemic and mucosal antibody responses in mice immunized intranasally with aluminium-non-adsorbed diphtheria toroid together with recombinant cholera toxin B subunit as an adjuvant, VACCINE, 18(7-8), 1999, pp. 743-751
Citations number
29
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264410X → ACNP
Volume
18
Issue
7-8
Year of publication
1999
Pages
743 - 751
Database
ISI
SICI code
0264-410X(19991112)18:7-8<743:IOSAMA>2.0.ZU;2-8
Abstract
Nasal mucosal immunization is very attractive for vaccination to prevent va rious bacterial and viral infectious diseases because of induction of syste mic and mucosal immune responses. The aim of the present study was to inves tigate the possibility of changing the immunization procedure of diphtheria toxoid (D-T) from intramuscular or subcutaneous injection to intranasal ad ministration. Intranasal immunization with aluminium-non-adsorbed diphtheri a toroid (nD(T)) together with recombinant cholera toxin B subunit (rCTB, 1 0 mu g) induced, at a concentration of 5 Lf, high levels of serum DT(-)spec ific IgG antibody responses and high or moderate levels of the specific IgA antibody responses in all mice and only a slight level of the specific IgE antibody responses in some mice. Furthermore, sufficiently high diphtheria antitoxin titres more than 0.1 international units (IU) ml(-1) were obtain ed from mice which showed high levels of serum D-T-specific IgG antibody re sponses. Under the same experimental conditions, induction of significant l evels of mucosal D-T-specific IgA antibody responses occurred in the nasal cavity, the lung, the saliva and vaginal secretions and the small and large intestines of all mice, although there were different titres between indiv idual mice. Similar results were also obtained with rCTB-specific serum IgG and IgA and mucosal IgA antibody responses; serum rCTB-specific IgE antibo dy titres were not detected. These results show that intranasal administrat ion of nD(T) with rCTB must be a very useful means for vaccination against diphtheria. (C) 1999 Elsevier Science Ltd. All rights reserved.